Application note Comparative analysis of stromal cell isolation from adipose tissue using Roche Liberase MNP-S* and Worthington Collagenase I
نویسنده
چکیده
September 2016 S. Merfeld-Clauss, D.O. Traktuev, K. L. March The idea of cell therapy using the systemic or local injection of stem/progenitor cells in the area of injury to treat multiple chronic disorders received close attention in the last decade [1]. Bone marrow-derived mesenchymal stem cells (BM-MSC) and blood-derived endothelial progenitor cells were among the first cell types that were evaluated in regard to their therapeutic potentials [2, 3, 4].
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Optimization of Roche Liberase TM Research Grade in the Enzymatic Digestion of Human Adipose Tissue for the Isolation of Stem and Regenerative Cells
Adipose-derived stem cells are increasingly targeted as an autologous source of multipotent cells in regenerative medicine. Specifically, reconstructive lipotransfer procedures combine freshly harvested adipose tissue with ADSCs to improve vascularization and tissue grafting [1]. Furthermore, ADSCs offer a wide array of potential uses in tissue reengineering and cell therapies including wound h...
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